[Home ] [Archive]   [ فارسی ]  
:: Volume 22, Issue 2 (Iranian Journal of Ophthalmology 2010) ::
2010, 22(2): 67-71 Back to browse issues page
Induction of Alpha-Crystallins Expression in Umbilical Cord Mesenchymal Stem Cells
Masoud Maleki Dr. *, Kazem Parivar Dr., Mohammad Nabiyouni Dr., Parichehr Yaghmaei Dr., Mohammad Naji
, maleki.masoud@gmail.com
Abstract:   (15396 Views)

Purpose : Our aim was to isolate mesenchymal stem cells (MSCs) from mouse umbilical cord and culture them with vitreous body to investigate their capacity to differentiate into lens fiber cells, crystallins are expressed.

Methods : Wharton's jelly is the appropriate source of MSCs. After isolation, the cells were cultured and alkaline phosphatase detection kit was used for staining undifferentiated umbilical cord-MSCs. In the experimental groups, MSCs were plated in the maintenance medium supplemented with bovine vitreous body (1:1, 3:1 v/v) for induction up to 10 days. Light microscopy and immunostaining methods used to assess differentiation of the cells.

Results : Mouse umbilical cord-MSCs have alkaline phosphatase activity and vitreous body can induce them to differentiate into the lens fiber cells.

Conclusion : Lens fiber cells can be produced by umbilical cord-MSCs derived from mouse. MSCs have the potential to be useful source for stem cell researchers.


Iranian Journal of Ophthalmology 201022(2):67-71 © 2010 by the Iranian Society of Ophthalmology
Keywords: Crystallin, Lens Fiber Cells, Mesenchymal Stem Cells, Vitreous Body
Full-Text [PDF 316 kb]   (3570 Downloads)    
Type of Study: Original | Subject: General
Send email to the article author

Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Maleki M, Parivar K, Nabiyouni M, Yaghmaei P, Naji M. Induction of Alpha-Crystallins Expression in Umbilical Cord Mesenchymal Stem Cells. Iranian Journal of Ophthalmology. 2010; 22 (2) :67-71
URL: http://irjo.org/article-1-342-en.html

Volume 22, Issue 2 (Iranian Journal of Ophthalmology 2010) Back to browse issues page
مجله چشم پزشکی ایران Iranian Journal of Ophthalmology
Persian site map - English site map - Created in 0.07 seconds with 31 queries by YEKTAWEB 3876