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:: Volume 25, Issue 1 (Iranian Journal of Ophthalmology 2013) ::
2013, 25(1): 3-7 Back to browse issues page
Special Report: Long-Term Cryoprotectant-Augmented Deep Freezing of the Animal Whole Globe for the Maintenance of Corneal Clarity
Arash Mazouri Dr., S-Farzad Mohammadi Dr. * , Nazanin Rahman-A Dr., Mahmoud Jabbarvand Dr.
, sfmohamm@razi.tums.ac.ir
Abstract:   (8068 Views)

Purpose : Round-the-clock on-demand availability and corneal cloudiness are the two major problems with animal eyes for surgical practice. The purpose of this investigation is to assess the usability of long-term deep freezing for the preservation of enucleated sheep eyes and the adjunctive role of intracameral cryoprotective agent on corneal clarity.

Methods : Thirty-two enucleated fresh sheep eyes were obtained from the slaughterhouse. With an insulin syringe, 0.5 mL of aqueous was tapped from the 16 right globes it was replaced by an equivalent intracameral cryoprotection solution. Treated eyes were kept in receptacles filled with 1 cc of the same cryoprotectant and stored in a deep freezer (-80˚C). Left eyes were served as controls (untreated globes) and stored in the same condition. Six months later, globes were defrosted and corneal clarity was compared in a paired and masked fashion.

Results : All treated globes had clearer corneas as compared with untreated globes and they were all successfully applied for anterior segment surgical practice.

Conclusion : Long-term deep freezing with adjuvant intracameral cryoprotectant is effective for the preservation of corneal clarity of animal whole globe. This allows round-the-clock on-demand availability of animal eyes with clear corneas for surgical practice in ophthalmology skill labs.

  

Iranian Journal of Ophthalmology 201325(1):3-7 © 2013 by the Iranian Society of Ophthalmology

Keywords: Ophthalmology Training, Animal Eye, Eye Model, Skill Lab, Corneal Clarity, Globe Preservation
Full-Text [PDF 371 kb]   (2439 Downloads)    
Type of Study: Original | Subject: Anterior segment
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Mazouri A, Mohammadi S, Rahman-A N, Jabbarvand M. Special Report: Long-Term Cryoprotectant-Augmented Deep Freezing of the Animal Whole Globe for the Maintenance of Corneal Clarity. Iranian Journal of Ophthalmology. 2013; 25 (1) :3-7
URL: http://irjo.org/article-1-778-en.html


Volume 25, Issue 1 (Iranian Journal of Ophthalmology 2013) Back to browse issues page
مجله چشم پزشکی ایران Iranian Journal of Ophthalmology
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