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:: Volume 26, Issue 2 (Iranian Journal of Ophthalmology 2014) ::
2014, 26(2): 97-101 Back to browse issues page
Isolation and Genotyping of Acanthamoeba Strains from Corneal Scraps
MirMostafa Ghamilouie * , Zarrintaj Valadkhani Dr., Firoozeh Rahimi Dr., Fariba Khoshzaban Dr., Zohreh Aghighi , Nayerreh Hassan
Abstract:   (3991 Views)

 Purpose: To determine the prevalence and genotype of Acanthamoeba keratitis (AK) in patients referred to Farabi referral eye center

 Methods: In this study, corneal scraping specimens that obtained from keratitis patients examined for Acanthamoeba and its genotype identifications. Direct smears, cultivation in non-nutrient agar (NNA) followed by PCR were chosen as the basic diagnostic methods. The positive patients checked by confocal microscopy. For genotypic identifications of isolates from keratitis patients, DF3 region of rRNA gene amplified in PCR.

 Results: Five clinical specimens (5.6%) out of 89 collected samples showed positive by culture and PCR analysis. Then the results compared with confocal microscopy and all five patients confirmed as AK. The statistical analysis showed significant relation between infection with Acanthamoeba and wearing contact lenses (p=0.001). A logistic regression analysis showed, there is an inverse weak statistical relation between age and infection (p=0.07). Sequence analysis demonstrated that T4 is the predominant genotype in these isolates and the primary genotype in AK. All cases were unilateral and pain, redness, tearing, and light sensitivity were the most common clinical symptoms among them.

 Conclusion: Genotypic identification of the microorganism helps epidemiologists and parasitologists to study the transmission routes of infection in different areas. The results of this study also showed that NNA as diagnostic and economical method could be used in areas with low ophthalmic equipment such as confocal microscopy, but it is time consuming.

  

 Iranian Journal of Ophthalmology 201426(2):97-101 © 2014 by the Iranian Society of Ophthalmology

Keywords: Acanthamoeba Keratitis, Epidemiology, DF3, Genotype
Full-Text [PDF 325 kb]   (1296 Downloads)    
Type of Study: Original | Subject: Anterior segment
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Ghamilouie M, Valadkhani Z, Rahimi F, Khoshzaban F, Aghighi Z, Hassan N. Isolation and Genotyping of Acanthamoeba Strains from Corneal Scraps. Iranian Journal of Ophthalmology. 2014; 26 (2) :97-101
URL: http://irjo.org/article-1-933-en.html


Volume 26, Issue 2 (Iranian Journal of Ophthalmology 2014) Back to browse issues page
مجله چشم پزشکی ایران Iranian Journal of Ophthalmology
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